Journal: Journal of gastroenterology and hepatology
Article Title: Insulin resistance in experimental alcohol-induced liver disease
doi: 10.1111/j.1440-1746.2008.05339.x
Figure Lengend Snippet: Chronic ethanol feeding inhibits insulin-responsive gene expression in the liver. Adult Long-Evans rats were fed with isocaloric control or ethanol-containing liquid diets for 8 weeks. Liver protein homogenates were used to measure AAH (A85G6 antibody), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), and the p85 subunit of PI3 kinase (loading control) protein expression by (a) Western blot analysis (n = 6 per group) or (b–d) ELISA (n = 12 per group). In panel a, positions of molecular weight standards are shown at the left, and representative results are shown. (b) AAH, (c) GAPDH, and (d) p85-PI3 kinase protein levels were also measured direct by ELISA using HRP-conjugated secondary antibody and ECL reagents. Luminescence (relative light units; RLU) was measured in a Top Count machine. Graphs depict the mean ± SEM of results. Inter-group statistical comparisons were made using Student’s t-tests. Significant P-values are indicated over the bar graphs. Con, control; EtOH ethanol.
Article Snippet: Adult male Long-Evans rats were pair-fed ethanol-containing ( n = 12) or isocaloric control ( n = 13) liquid diets (BioServ, Frenchtown, NJ, USA) for 8 weeks.
Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Molecular Weight, Direct ELISA